Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1882302..1882804 Replicon chromosome
Accession NZ_CP091773
Organism Escherichia coli strain SWYH.B349aT

Toxin (Protein)


Gene name yoeB Uniprot ID V0THH9
Locus tag L6V99_RS09080 Protein ID WP_023141665.1
Coordinates 1882550..1882804 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag L6V99_RS09075 Protein ID WP_001259255.1
Coordinates 1882302..1882553 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6V99_RS09050 (1877480) 1877480..1878547 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
L6V99_RS09055 (1878547) 1878547..1879617 - 1071 WP_159139312.1 histidinol-phosphate transaminase -
L6V99_RS09060 (1879614) 1879614..1880918 - 1305 WP_033865584.1 histidinol dehydrogenase -
L6V99_RS09065 (1880924) 1880924..1881823 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
L6V99_RS09070 (1881969) 1881969..1882019 - 51 WP_001364200.1 his operon leader peptide -
L6V99_RS09075 (1882302) 1882302..1882553 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
L6V99_RS09080 (1882550) 1882550..1882804 + 255 WP_023141665.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
L6V99_RS09085 (1882887) 1882887..1883711 + 825 WP_000754758.1 SDR family oxidoreductase -
L6V99_RS09090 (1883757) 1883757..1884686 + 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
L6V99_RS09095 (1884901) 1884901..1884963 + 63 WP_010723108.1 membrane protein YoeI -
L6V99_RS09100 (1884953) 1884953..1886311 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
L6V99_RS09105 (1886490) 1886490..1887548 + 1059 WP_029397759.1 thiosulfate utilization transporter TsuA/YeeE -
L6V99_RS09110 (1887562) 1887562..1887789 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10229.67 Da        Isoelectric Point: 7.8733

>T234744 WP_023141665.1 NZ_CP091773:1882550-1882804 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFECKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T234744 NZ_LR134079:c5264887-5264576 [Escherichia coli]
ATACTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT234744 WP_001259255.1 NZ_CP091773:1882302-1882553 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT234744 NZ_LR134079:c5264575-5264285 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0THH9

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References