Detailed information of TA system TA234742

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1123671..1124398
• Replicon: chromosome
• Accession: NZ_CP091773
• Organism: Escherichia coli strain SWYH.B349aT

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: L6V99_RS05465
• Protein ID: WP_000547564.1
• Coordinates: 1123671..1123982 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L6V99_RS05470
• Protein ID: WP_000126294.1
• Coordinates: 1123979..1124398 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6V99_RS05435 (1118813)	1118813..1120522	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
L6V99_RS05440 (1120532)	1120532..1121074	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
L6V99_RS05445 (1121074)	1121074..1121841	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
L6V99_RS05450 (1121838)	1121838..1122248	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
L6V99_RS05455 (1122241)	1122241..1122711	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
L6V99_RS05460 (1122736)	1122736..1123497	+	762	WP_001026446.1	hypothetical protein	-
L6V99_RS05465 (1123671)	1123671..1123982	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
L6V99_RS05470 (1123979)	1123979..1124398	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
L6V99_RS05475 (1124512)	1124512..1125936	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
L6V99_RS05480 (1125945)	1125945..1127402	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
L6V99_RS05485 (1127662)	1127662..1128672	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
L6V99_RS05490 (1128821)	1128821..1129348	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T234742 WP_000547564.1 NZ_CP091773:1123671-1123982 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T234742 NZ_LR134079:4902457-4902834 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT234742 WP_000126294.1 NZ_CP091773:1123979-1124398 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT234742 NZ_LR134079:4902000-4902380 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGGGGCCGGT
TCAACAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5