Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 626361..627160 Replicon chromosome
Accession NZ_CP091773
Organism Escherichia coli strain SWYH.B349aT

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag L6V99_RS03035 Protein ID WP_000347251.1
Coordinates 626361..626825 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag L6V99_RS03040 Protein ID WP_001307405.1
Coordinates 626825..627160 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6V99_RS03005 (621362) 621362..621796 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
L6V99_RS03010 (621814) 621814..622692 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
L6V99_RS03015 (622682) 622682..623461 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
L6V99_RS03020 (623472) 623472..623945 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
L6V99_RS03025 (623968) 623968..625248 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
L6V99_RS03030 (625497) 625497..626306 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
L6V99_RS03035 (626361) 626361..626825 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
L6V99_RS03040 (626825) 626825..627160 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
L6V99_RS03045 (627309) 627309..628880 - 1572 WP_001273761.1 galactarate dehydratase -
L6V99_RS03050 (629255) 629255..630589 + 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
L6V99_RS03055 (630605) 630605..631375 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T234738 WP_000347251.1 NZ_CP091773:c626825-626361 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T234738 NZ_LR134079:4248845-4249123 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATATTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT234738 WP_001307405.1 NZ_CP091773:c627160-626825 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT234738 NZ_LR134079:4248582-4248842 [Escherichia coli]
ATGGCTGCAAATGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References