Detailed information of TA system TA234725

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39603..39857
• Replicon: plasmid p2-LC-1302-2020
• Accession: NZ_CP091758
• Organism: Escherichia coli strain LC-1302-2020

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: L6507_RS26145
• Protein ID: WP_001312851.1
• Coordinates: 39708..39857 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	39603..39664 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6507_RS26120 (36778)	36778..37524	+	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
L6507_RS26125 (37579)	37579..38139	+	561	WP_000139325.1	fertility inhibition protein FinO	-
L6507_RS26130 (38271)	38271..38483	+	213	WP_001301409.1	ANR family transcriptional regulator	-
L6507_RS26135 (38729)	38729..38815	+	87	Protein_40	endonuclease	-
L6507_RS26140 (38996)	38996..39307	-	312	WP_000802277.1	hypothetical protein	-
- (39603)	39603..39664	-	62	NuclAT_0	-	Antitoxin
- (39603)	39603..39664	-	62	NuclAT_0	-	Antitoxin
- (39603)	39603..39664	-	62	NuclAT_0	-	Antitoxin
- (39603)	39603..39664	-	62	NuclAT_0	-	Antitoxin
L6507_RS26145 (39708)	39708..39857	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
L6507_RS26150 (40141)	40141..40398	+	258	WP_000083845.1	replication regulatory protein RepA	-
L6507_RS26155 (40501)	40501..40687	+	187	Protein_44	protein CopA/IncA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..40699	40699

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T234725 WP_001312851.1 NZ_CP091758:39708-39857 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T234725 NZ_LR134079:c627644-627180 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT234725 NZ_CP091758:c39664-39603 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin