234720

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4543951..4544448 Replicon chromosome
Accession NZ_CP091756
Organism Escherichia coli strain LC-1302-2020

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag L6507_RS22125 Protein ID WP_001173343.1
Coordinates 4543951..4544124 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag L6507_RS22130 Protein ID WP_000398619.1
Coordinates 4544152..4544448 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6507_RS22115 (4540621) 4540621..4542078 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
L6507_RS22120 (4542315) 4542315..4543832 + 1518 WP_001295074.1 lysine--tRNA ligase -
L6507_RS22125 (4543951) 4543951..4544124 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
L6507_RS22130 (4544152) 4544152..4544448 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
L6507_RS22135 (4544675) 4544675..4544947 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
L6507_RS22140 (4544959) 4544959..4545189 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
L6507_RS22145 (4545370) 4545370..4547001 + 1632 WP_001216477.1 sensor histidine kinase -
L6507_RS22150 (4546998) 4546998..4547717 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4530190..4544448 14258


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T234720 WP_001173343.1 NZ_CP091756:c4544124-4543951 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T234720 NZ_LR134079:208335-208442 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT234720 WP_000398619.1 NZ_CP091756:c4544448-4544152 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT234720 NZ_LR134079:c208286-208221 [Escherichia coli]
GTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References