Detailed information of TA system TA023472

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HipB
• Location: 1538400..1539073
• Replicon: chromosome
• Accession: NC_011830
• Organism: Desulfitobacterium hafniense DCB-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B8FNX0
• Locus tag: DHAF_RS07200
• Protein ID: WP_015943428.1
• Coordinates: 1538400..1538759 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DHAF_RS07205
• Protein ID: WP_015943429.1
• Coordinates: 1538762..1539073 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DHAF_RS07180	1534004..1534549	+	546	WP_005817255.1	hypoxanthine phosphoribosyltransferase	-
DHAF_RS07185	1534661..1536193	+	1533	WP_015943425.1	glutamine-hydrolyzing GMP synthase	-
DHAF_RS07190	1536346..1537299	+	954	WP_015943426.1	tyrosine-type recombinase/integrase	-
DHAF_RS07195	1537439..1538119	+	681	WP_015943427.1	hypothetical protein	-
DHAF_RS07200	1538400..1538759	+	360	WP_015943428.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DHAF_RS07205	1538762..1539073	+	312	WP_015943429.1	helix-turn-helix transcriptional regulator	Antitoxin
DHAF_RS07210	1539051..1539488	+	438	WP_015943430.1	hypothetical protein	-
DHAF_RS25665	1539633..1539782	+	150	WP_158304529.1	helix-turn-helix domain-containing protein	-
DHAF_RS07225	1541273..1541461	+	189	WP_015943432.1	hypothetical protein	-
DHAF_RS25960	1541466..1541624	+	159	WP_015943433.1	hypothetical protein	-
DHAF_RS07230	1542129..1543778	+	1650	WP_076611670.1	IS1634-like element ISDha9 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	groEL	1512257..1575217	62960

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-87)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 14359.49 Da
• Isoelectric Point: 9.8662

>T23472 WP_015943428.1 NC_011830:1538400-1538759 [Desulfitobacterium hafniense DCB-2]
VFKVIIYEDKNGKSSIADYIAELDEKANTSKDSRVRLKKIYQYFELLKIYGTRAGLPSSKYIEEDIWELRPTNDRFFYVY
WKNDTFVILHHYIKKTQKAPRQEIELAKRNLKDFLERSK

Nucleotide

• Length: 360 bp

>T23472 NC_011830:1538400-1538759 [Desulfitobacterium hafniense DCB-2]
GTGTTCAAAGTAATAATTTACGAGGATAAAAATGGTAAATCCTCGATTGCTGATTATATTGCAGAGCTGGATGAAAAGGC
TAACACCAGTAAAGATAGTCGGGTAAGGCTGAAAAAAATATATCAGTATTTTGAACTGTTAAAAATATATGGTACTCGTG
CAGGGTTACCATCTTCAAAATATATTGAGGAGGATATTTGGGAGCTTCGACCTACTAATGATCGATTTTTCTATGTGTAC
TGGAAGAACGATACATTTGTTATTCTTCATCACTACATAAAGAAGACTCAAAAAGCGCCTCGACAGGAAATTGAACTAGC
AAAGAGGAACTTAAAAGACTTCTTGGAAAGGAGTAAATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11511.09 Da
• Isoelectric Point: 4.8836

>AT23472 WP_015943429.1 NC_011830:1538762-1539073 [Desulfitobacterium hafniense DCB-2]
MAIGRDWDDIKKNLSSLTQEDKDEIDFQVKLIGEILAARKEKGITQAELDSLCGVKQTHIARIENNNTDPQLSTIFKILR
PLGKTLAVVDIPETLDHASDAHF

Nucleotide

• Length: 312 bp

>AT23472 NC_011830:1538762-1539073 [Desulfitobacterium hafniense DCB-2]
ATGGCAATTGGTAGAGATTGGGACGATATTAAAAAAAATCTGTCTTCTTTAACACAGGAGGATAAAGACGAGATTGATTT
TCAAGTAAAATTGATTGGCGAGATTTTAGCAGCTAGGAAAGAAAAAGGAATAACTCAAGCAGAACTTGATTCTCTTTGTG
GAGTGAAGCAAACTCATATAGCCCGAATAGAAAATAATAACACGGATCCACAACTTTCTACTATTTTTAAAATCTTGCGC
CCACTTGGAAAAACTCTGGCTGTAGTGGATATACCAGAGACGTTAGATCATGCTTCTGATGCACATTTCTAA

Structures

Toxin

• AlphaFold DB: B8FNX0