Detailed information of TA system TA234717

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3889176..3889870
• Replicon: chromosome
• Accession: NZ_CP091756
• Organism: Escherichia coli strain LC-1302-2020

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A3P5GWS5
• Locus tag: L6507_RS18990
• Protein ID: WP_001263488.1
• Coordinates: 3889176..3889574 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: L6507_RS18995
• Protein ID: WP_000554758.1
• Coordinates: 3889577..3889870 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3884764)	3884764..3884844	-	81	NuclAT_12	-	-
- (3884764)	3884764..3884844	-	81	NuclAT_12	-	-
- (3884764)	3884764..3884844	-	81	NuclAT_12	-	-
- (3884764)	3884764..3884844	-	81	NuclAT_12	-	-
L6507_RS18965 (3885440)	3885440..3885898	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
L6507_RS18970 (3886159)	3886159..3887616	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
L6507_RS18975 (3887673)	3887673..3888194	-	522	Protein_3710	peptide chain release factor H	-
L6507_RS18980 (3888190)	3888190..3888396	-	207	Protein_3711	RtcB family protein	-
L6507_RS18985 (3888714)	3888714..3889166	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
L6507_RS18990 (3889176)	3889176..3889574	-	399	WP_001263488.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
L6507_RS18995 (3889577)	3889577..3889870	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
L6507_RS19000 (3889922)	3889922..3890977	-	1056	WP_001226164.1	DNA polymerase IV	-
L6507_RS19005 (3891048)	3891048..3891833	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
L6507_RS19010 (3891805)	3891805..3893517	+	1713	Protein_3717	flagellar biosynthesis protein FlhA	-
L6507_RS19015 (3893741)	3893741..3894238	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3888714..3904456	15742

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15559.90 Da
• Isoelectric Point: 6.9798

>T234717 WP_001263488.1 NZ_CP091756:c3889574-3889176 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQEAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T234717 NZ_LR134075:c3911606-3911319 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT234717 WP_000554758.1 NZ_CP091756:c3889870-3889577 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT234717 NZ_LR134075:c3911332-3911030 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A3P5GWS5

Antitoxin

• AlphaFold DB: S1QAE3