Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 645844..646643 Replicon chromosome
Accession NZ_CP091756
Organism Escherichia coli strain LC-1302-2020

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag L6507_RS03125 Protein ID WP_000347275.1
Coordinates 645844..646308 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag L6507_RS03130 Protein ID WP_001307405.1
Coordinates 646308..646643 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6507_RS03095 (640845) 640845..641279 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
L6507_RS03100 (641297) 641297..642175 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
L6507_RS03105 (642165) 642165..642944 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
L6507_RS03110 (642955) 642955..643428 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
L6507_RS03115 (643451) 643451..644731 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
L6507_RS03120 (644980) 644980..645789 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
L6507_RS03125 (645844) 645844..646308 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
L6507_RS03130 (646308) 646308..646643 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
L6507_RS03135 (646792) 646792..648363 - 1572 WP_001273753.1 galactarate dehydratase -
L6507_RS03140 (648738) 648738..650072 + 1335 WP_115725679.1 galactarate/glucarate/glycerate transporter GarP -
L6507_RS03145 (650088) 650088..650858 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T234699 WP_000347275.1 NZ_CP091756:c646308-645844 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T234699 NZ_LR134075:204143-204250 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT234699 WP_001307405.1 NZ_CP091756:c646643-646308 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT234699 NZ_LR134075:c204095-204029 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References