Detailed information of TA system TA234683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1965059..1965719
• Replicon: chromosome
• Accession: NZ_CP091752
• Organism: Klebsiella grimontii strain LC-1303-2020

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A285B6D8
• Locus tag: L6506_RS09250
• Protein ID: WP_004132737.1
• Coordinates: 1965059..1965412 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A9E1DQ94
• Locus tag: L6506_RS09255
• Protein ID: WP_004132739.1
• Coordinates: 1965417..1965719 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6506_RS09225 (L6506_09225)	1960443..1961210	-	768	WP_004132727.1	molybdopterin-dependent oxidoreductase	-
L6506_RS09230 (L6506_09230)	1961200..1961808	-	609	WP_004132729.1	cytochrome b/b6 domain-containing protein	-
L6506_RS09235 (L6506_09235)	1961823..1962449	-	627	WP_004132731.1	hypothetical protein	-
L6506_RS09240 (L6506_09240)	1962589..1963257	+	669	WP_004132733.1	heavy metal response regulator transcription factor	-
L6506_RS09245 (L6506_09245)	1963257..1964675	+	1419	WP_154930148.1	heavy metal sensor histidine kinase	-
L6506_RS09250 (L6506_09250)	1965059..1965412	+	354	WP_004132737.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L6506_RS09255 (L6506_09255)	1965417..1965719	+	303	WP_004132739.1	XRE family transcriptional regulator	Antitoxin
L6506_RS09260 (L6506_09260)	1965810..1965980	+	171	WP_165909793.1	helix-turn-helix domain-containing protein	-
L6506_RS09265 (L6506_09265)	1966103..1966306	+	204	Protein_1816	hypothetical protein	-
L6506_RS09270 (L6506_09270)	1966307..1966640	+	334	Protein_1817	type II toxin-antitoxin system PemK/MazF family toxin	-
L6506_RS09280 (L6506_09280)	1966963..1968420	+	1458	WP_004136541.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13554.49 Da
• Isoelectric Point: 10.0045

>T234683 WP_004132737.1 NZ_CP091752:1965059-1965412 [Klebsiella grimontii]
VWMIKTTDTFERWFTSFNDTDRASVLAALLVLREKGPGLSRPNADTLRGSRYSNMKELRIQSRGEPIRAFFAFDPARTGI
VLCAGNKVGNEKRFYDEMLPVAEREFTNWLKTFKEKE

Nucleotide

• Length: 354 bp

>T234683 NZ_LR134031:1107715-1108050 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11072.83 Da
• Isoelectric Point: 5.7424

>AT234683 WP_004132739.1 NZ_CP091752:1965417-1965719 [Klebsiella grimontii]
MGRTLEQLMADEKPEVVADARAMAADILLNIHLAELREKVQKTQVEMAQALGIRQPTVAGMEKPGRDLKLSTLKRYVEAT
GGKLRLDVELPDGSHYGFEL

Nucleotide

• Length: 303 bp

>AT234683 NZ_LR134031:1107467-1107715 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A285B6D8