Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4136427..4137226 Replicon chromosome
Accession NZ_CP091712
Organism Escherichia coli strain KFu014

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag L6L57_RS19730 Protein ID WP_000347273.1
Coordinates 4136762..4137226 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V0TI39
Locus tag L6L57_RS19725 Protein ID WP_021547164.1
Coordinates 4136427..4136762 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L57_RS19710 (4132212) 4132212..4132982 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
L6L57_RS19715 (4132998) 4132998..4134332 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
L6L57_RS19720 (4134707) 4134707..4136278 + 1572 WP_001273756.1 galactarate dehydratase -
L6L57_RS19725 (4136427) 4136427..4136762 + 336 WP_021547164.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
L6L57_RS19730 (4136762) 4136762..4137226 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
L6L57_RS19735 (4137281) 4137281..4138090 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
L6L57_RS19740 (4138339) 4138339..4139619 + 1281 WP_000681958.1 tagatose-bisphosphate aldolase subunit KbaZ -
L6L57_RS19745 (4139642) 4139642..4140115 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
L6L57_RS19750 (4140126) 4140126..4140905 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
L6L57_RS19755 (4140895) 4140895..4141773 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
L6L57_RS19760 (4141791) 4141791..4142225 + 435 WP_000948834.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4125944..4137226 11282


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T234679 WP_000347273.1 NZ_CP091712:4136762-4137226 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T234679 NZ_LR134031:189359-189466 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCACGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12309.86 Da        Isoelectric Point: 4.5392

>AT234679 WP_021547164.1 NZ_CP091712:4136427-4136762 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT234679 NZ_LR134031:c189310-189245 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB V0TI39

References