Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 68298..68551 Replicon plasmid pKGu016_2
Accession NZ_CP091706
Organism Escherichia coli strain KGu016

Toxin (Protein)


Gene name srnB Uniprot ID A0A148HBD8
Locus tag L6L59_RS25670 Protein ID WP_001336447.1
Coordinates 68402..68551 (+) Length 50 a.a.

Antitoxin (RNA)


Gene name srnC
Locus tag -
Coordinates 68298..68354 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L59_RS25640 (64345) 64345..64668 + 324 Protein_85 type-F conjugative transfer system pilin acetylase TraX -
L6L59_RS25645 (64701) 64701..66314 - 1614 WP_150329183.1 IS66-like element ISEc23 family transposase -
L6L59_RS25650 (66345) 66345..66695 - 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
L6L59_RS25655 (66692) 66692..67117 - 426 WP_000422741.1 transposase -
L6L59_RS25660 (67395) 67395..67613 + 219 WP_113365647.1 hypothetical protein -
L6L59_RS25665 (67766) 67766..68203 + 438 WP_000872609.1 hypothetical protein -
- (68298) 68298..68354 - 57 NuclAT_1 - Antitoxin
- (68298) 68298..68354 - 57 NuclAT_1 - Antitoxin
- (68298) 68298..68354 - 57 NuclAT_1 - Antitoxin
- (68298) 68298..68354 - 57 NuclAT_1 - Antitoxin
L6L59_RS25670 (68402) 68402..68551 + 150 WP_001336447.1 Hok/Gef family protein Toxin
L6L59_RS25675 (68836) 68836..69093 + 258 WP_000084404.1 replication regulatory protein RepA -
L6L59_RS25680 (69198) 69198..69383 + 186 WP_012615301.1 plasmid copy control protein CopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..69395 69395


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(4-45)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5572.69 Da        Isoelectric Point: 8.7678

>T234655 WP_001336447.1 NZ_CP091706:68402-68551 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 150 bp

>T234655 NZ_LR133996:2142237-2142419 [Shimwellia blattae]
CTGAAGAGCTCGGAACTGATATCACTACTGGAGAAAAATGGCTGGGTCCTGGAGCGCATCAAAGGCAGCCATCACCAGTT
CTCCCATCCCGATTTTGCTATTGTCATTACTGTTCCGCATCCCAGAAAAGACGTGAAGCCAGGAACGCTCCGTCAGATCC
TGAAGAATGCGAAGCTAAAATGA

Antitoxin


Download         Length: 57 bp

>AT234655 NZ_CP091706:c68354-68298 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A148HBD8

Antitoxin

Download structure file

References