Detailed information of TA system TA234652

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 25362..25631
• Replicon: plasmid pKGu016_2
• Accession: NZ_CP091706
• Organism: Escherichia coli strain KGu016

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: L6L59_RS25400
• Protein ID: WP_001372321.1
• Coordinates: 25506..25631 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	25362..25427 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L59_RS25360	20392..20829	-	438	Protein_29	hypothetical protein	-
L6L59_RS25365	21131..21658	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
L6L59_RS25370	21716..21949	+	234	WP_000006003.1	DUF905 family protein	-
L6L59_RS25375	22010..23974	+	1965	WP_000117316.1	ParB/RepB/Spo0J family partition protein	-
L6L59_RS25380	24043..24477	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
L6L59_RS25385	24474..25236	+	763	Protein_34	plasmid SOS inhibition protein A	-
-	25205..25429	+	225	NuclAT_0	-	-
-	25205..25429	+	225	NuclAT_0	-	-
-	25205..25429	+	225	NuclAT_0	-	-
-	25205..25429	+	225	NuclAT_0	-	-
L6L59_RS25390	25214..25393	-	180	WP_001309233.1	hypothetical protein	-
-	25362..25427	-	66	-	-	Antitoxin
L6L59_RS25395	25415..25564	+	150	Protein_36	plasmid maintenance protein Mok	-
L6L59_RS25400	25506..25631	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
L6L59_RS25405	25950..26246	-	297	Protein_38	hypothetical protein	-
L6L59_RS25410	26546..26842	+	297	WP_001272251.1	hypothetical protein	-
L6L59_RS25415	26953..27774	+	822	WP_021549731.1	DUF932 domain-containing protein	-
L6L59_RS25420	28071..28718	-	648	Protein_41	transglycosylase SLT domain-containing protein	-
L6L59_RS25425	28994..29377	+	384	WP_001151529.1	conjugal transfer relaxosome DNA-binding protein TraM	-
L6L59_RS25430	29569..30216	+	648	WP_000332523.1	transcriptional regulator TraJ family protein	-
L6L59_RS25435	30336..30563	+	228	WP_000589556.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..69395	69395

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T234652 WP_001372321.1 NZ_CP091706:25506-25631 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T234652 NZ_LR133996:1596195-1596527 [Shimwellia blattae]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTGGATCCCACAGCCGGACACGAACAAAGCGGGAAACGCCCGGTGCTTAT
CGTATCGAAGGCCTCGTTCAACACGTTCACCCGGTTGCCCGTTGTTGTTCCTGTCACCAGCGGCGGGAATTTTGCCCGTA
CGGCTGGTTTTGCTGTTTCACTGGATGGTGCAGGGACGAAAACAACGGGCGTTATTCGCTGTGACCAGCCCAGAACTATC
GATATGGCGGCCAGAAATGGTAAGCGGCTGGAACGTCTGCCTGATGCTGTTGTGAATGAAGTGCTGGCCCGGCTGGAAGC
CATACTGAGCTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT234652 NZ_CP091706:c25427-25362 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin