Detailed information of TA system TA234645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4922796..4923398
• Replicon: chromosome
• Accession: NZ_CP091704
• Organism: Escherichia coli strain KGu016

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: L6L59_RS23655
• Protein ID: WP_000897305.1
• Coordinates: 4923087..4923398 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L6L59_RS23650
• Protein ID: WP_000356395.1
• Coordinates: 4922796..4923086 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L59_RS23615 (4918420)	4918420..4919322	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
L6L59_RS23620 (4919319)	4919319..4919954	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
L6L59_RS23625 (4919951)	4919951..4920880	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
L6L59_RS23630 (4921062)	4921062..4921304	-	243	WP_001309881.1	CopG family transcriptional regulator	-
L6L59_RS23635 (4921523)	4921523..4921741	-	219	WP_001295676.1	CopG family transcriptional regulator	-
L6L59_RS23640 (4922160)	4922160..4922438	-	279	WP_001315112.1	hypothetical protein	-
L6L59_RS23645 (4922490)	4922490..4922711	-	222	WP_001550354.1	hypothetical protein	-
L6L59_RS23650 (4922796)	4922796..4923086	-	291	WP_000356395.1	NadS family protein	Antitoxin
L6L59_RS23655 (4923087)	4923087..4923398	-	312	WP_000897305.1	hypothetical protein	Toxin
L6L59_RS23660 (4923627)	4923627..4924535	+	909	WP_001550353.1	alpha/beta hydrolase	-
L6L59_RS23665 (4924703)	4924703..4925617	-	915	WP_109553727.1	transposase	-
L6L59_RS23670 (4925630)	4925630..4926517	-	888	Protein_4639	hypothetical protein	-
L6L59_RS23675 (4926933)	4926933..4927874	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
L6L59_RS23680 (4927919)	4927919..4928356	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T234645 WP_000897305.1 NZ_CP091704:c4923398-4923087 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T234645 NZ_LR133964:c5016111-5015728 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT234645 WP_000356395.1 NZ_CP091704:c4923086-4922796 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT234645 NZ_LR133964:c5016353-5016111 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7