Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3952386..3953080 Replicon chromosome
Accession NZ_CP091704
Organism Escherichia coli strain KGu016

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag L6L59_RS19035 Protein ID WP_001263491.1
Coordinates 3952386..3952784 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag L6L59_RS19040 Protein ID WP_000554755.1
Coordinates 3952787..3953080 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3948215) 3948215..3948295 - 81 NuclAT_10 - -
- (3948215) 3948215..3948295 - 81 NuclAT_10 - -
- (3948215) 3948215..3948295 - 81 NuclAT_10 - -
- (3948215) 3948215..3948295 - 81 NuclAT_10 - -
L6L59_RS19005 (3947555) 3947555..3948799 - 1245 WP_000189541.1 esterase FrsA -
L6L59_RS19010 (3948891) 3948891..3949349 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
L6L59_RS19015 (3949610) 3949610..3951067 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
L6L59_RS19020 (3951124) 3951124..3951476 - 353 Protein_3733 peptide chain release factor H -
L6L59_RS19025 (3951472) 3951472..3951678 - 207 Protein_3734 RtcB family protein -
L6L59_RS19030 (3951924) 3951924..3952376 - 453 WP_019842500.1 GNAT family N-acetyltransferase -
L6L59_RS19035 (3952386) 3952386..3952784 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
L6L59_RS19040 (3952787) 3952787..3953080 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
L6L59_RS19045 (3953132) 3953132..3954187 - 1056 WP_001550655.1 DNA polymerase IV -
L6L59_RS19050 (3954258) 3954258..3955043 - 786 WP_019842510.1 putative lateral flagellar export/assembly protein LafU -
L6L59_RS19055 (3955015) 3955015..3956727 + 1713 Protein_3740 flagellar biosynthesis protein FlhA -
L6L59_RS19060 (3956832) 3956832..3957110 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
L6L59_RS19065 (3957103) 3957103..3957459 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3942322..3953080 10758


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T234639 WP_001263491.1 NZ_CP091704:c3952784-3952386 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T234639 NZ_LR133964:c2569920-2568598 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGTGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCGGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCGCCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT234639 WP_000554755.1 NZ_CP091704:c3953080-3952787 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT234639 NZ_LR133964:c2570186-2569920 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References