Detailed information of TA system TA234623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1174692..1175419
• Replicon: chromosome
• Accession: NZ_CP091704
• Organism: Escherichia coli strain KGu016

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: L6L59_RS05665
• Protein ID: WP_000547555.1
• Coordinates: 1174692..1175003 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L6L59_RS05670
• Protein ID: WP_000126294.1
• Coordinates: 1175000..1175419 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L59_RS05640 (1170627)	1170627..1172336	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
L6L59_RS05645 (1172346)	1172346..1172888	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
L6L59_RS05650 (1172888)	1172888..1173655	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
L6L59_RS05655 (1173652)	1173652..1174062	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
L6L59_RS05660 (1174055)	1174055..1174525	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
L6L59_RS05665 (1174692)	1174692..1175003	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
L6L59_RS05670 (1175000)	1175000..1175419	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
L6L59_RS05675 (1175498)	1175498..1176922	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
L6L59_RS05680 (1176931)	1176931..1178388	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
L6L59_RS05685 (1178648)	1178648..1179658	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
L6L59_RS05690 (1179807)	1179807..1180334	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T234623 WP_000547555.1 NZ_CP091704:1174692-1175003 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T234623 NZ_LR133917:c1878231-1878079 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGACTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT234623 WP_000126294.1 NZ_CP091704:1175000-1175419 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT234623 NZ_LR133917:c1878855-1878256 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: V0YLE2