Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 632838..633637 Replicon chromosome
Accession NZ_CP091704
Organism Escherichia coli strain KGu016

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag L6L59_RS03030 Protein ID WP_000347270.1
Coordinates 632838..633302 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag L6L59_RS03035 Protein ID WP_001551693.1
Coordinates 633302..633637 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L59_RS03000 (627839) 627839..628273 - 435 WP_000948837.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
L6L59_RS03005 (628291) 628291..629169 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
L6L59_RS03010 (629159) 629159..629938 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
L6L59_RS03015 (629949) 629949..630422 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
L6L59_RS03020 (630445) 630445..631725 - 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
L6L59_RS03025 (631974) 631974..632783 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
L6L59_RS03030 (632838) 632838..633302 - 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
L6L59_RS03035 (633302) 633302..633637 - 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
L6L59_RS03040 (633786) 633786..635357 - 1572 WP_001273738.1 galactarate dehydratase -
L6L59_RS03045 (635732) 635732..637066 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
L6L59_RS03050 (637082) 637082..637852 + 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T234618 WP_000347270.1 NZ_CP091704:c633302-632838 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

>T234618 NZ_LR132067:173937-174203 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAATCGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT234618 WP_001551693.1 NZ_CP091704:c633637-633302 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT234618 NZ_LR132067:173674-173937 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACAGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTAGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTGGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References