Detailed information of TA system TA234616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4380153..4380880
• Replicon: chromosome
• Accession: NZ_CP091703
• Organism: Escherichia coli strain KOr021

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: L6L60_RS20820
• Protein ID: WP_000547555.1
• Coordinates: 4380153..4380464 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L6L60_RS20825
• Protein ID: WP_000126294.1
• Coordinates: 4380461..4380880 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L60_RS20795 (4376088)	4376088..4377797	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
L6L60_RS20800 (4377807)	4377807..4378349	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
L6L60_RS20805 (4378349)	4378349..4379116	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
L6L60_RS20810 (4379113)	4379113..4379523	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
L6L60_RS20815 (4379516)	4379516..4379986	+	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
L6L60_RS20820 (4380153)	4380153..4380464	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
L6L60_RS20825 (4380461)	4380461..4380880	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
L6L60_RS20830 (4380959)	4380959..4382383	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
L6L60_RS20835 (4382392)	4382392..4383849	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
L6L60_RS20840 (4384109)	4384109..4385119	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
L6L60_RS20845 (4385268)	4385268..4385795	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T234616 WP_000547555.1 NZ_CP091703:4380153-4380464 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T234616 NZ_LR131273:704560-704988 [Tsukamurella paurometabola]
ATGAGTGACGTCGAGCTACCGGACGTCAACGTCCTCGTCGCCCTGCTGAGCGCCAACCACGTCCATCACACGACGGCACG
CGATTGGTTCGCATCGACGCCGCGGTTCGCCACGACACCGATCACCGAGGCCGGGCTCGTTCGGATGGCGCTGAACCGGG
CCGTCATGGGAACCGCGATCACCGCACAGCAGGCGCTCGCATCACTCGCCTCCCTCCGCGCGGACGACCGTGCCGAATTC
GTGCCGGACGAATCGTCGCTCGCCGCCCCGTCGATCGATCTCGTGGGGCTCGCCGGCCACAAGCAGGTCACCGACCTGCA
TCTCGTGAACCTGATGGCACGGCACTCCGGCGTGCTCGTCACCCTCGACACCAGGATCCGCCCCGTTCTCACTCCCGCCG
ACCAGCCGCTCGTCCGCGTCCTGCTCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT234616 WP_000126294.1 NZ_CP091703:4380461-4380880 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT234616 NZ_LR131273:704337-704567 [Tsukamurella paurometabola]
GTGCGTACGACCCTGGATATCAGCCCCCGCGTTCTCGCCGCCGCGCGAGCGCGGGTGAACGCCGGGCAGAACAAGAGCAT
CGGCGAGGCTGTGTCGGAGCTGGCCGCCCTCGGGCTCGAGACCGCCACGGCGAGCCGCCGACCGACCGATCACGGCCTCG
TCATGCTGCCGTCGGCACCCGATCACGTCATCACCAGCGAGATGGTCGAGGATGCGCTGCTCGATGAGTGA

Structures

Toxin

• AlphaFold DB: V0YLE2