Detailed information of TA system TA234609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3269489..3270091
• Replicon: chromosome
• Accession: NZ_CP091703
• Organism: Escherichia coli strain KOr021

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: L6L60_RS15500
• Protein ID: WP_000897305.1
• Coordinates: 3269489..3269800 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L6L60_RS15505
• Protein ID: WP_000356395.1
• Coordinates: 3269801..3270091 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L60_RS15475 (3264531)	3264531..3264968	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
L6L60_RS15480 (3265013)	3265013..3265954	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
L6L60_RS15485 (3266370)	3266370..3267257	+	888	Protein_3021	hypothetical protein	-
L6L60_RS15490 (3267270)	3267270..3268184	+	915	WP_109553727.1	transposase	-
L6L60_RS15495 (3268352)	3268352..3269260	-	909	WP_001550353.1	alpha/beta hydrolase	-
L6L60_RS15500 (3269489)	3269489..3269800	+	312	WP_000897305.1	hypothetical protein	Toxin
L6L60_RS15505 (3269801)	3269801..3270091	+	291	WP_000356395.1	NadS family protein	Antitoxin
L6L60_RS15510 (3270176)	3270176..3270397	+	222	WP_001550354.1	hypothetical protein	-
L6L60_RS15515 (3270449)	3270449..3270727	+	279	WP_001315112.1	hypothetical protein	-
L6L60_RS15520 (3271146)	3271146..3271364	+	219	WP_001295676.1	CopG family transcriptional regulator	-
L6L60_RS15525 (3271583)	3271583..3271825	+	243	WP_001309881.1	CopG family transcriptional regulator	-
L6L60_RS15530 (3272007)	3272007..3272936	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
L6L60_RS15535 (3272933)	3272933..3273568	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
L6L60_RS15540 (3273565)	3273565..3274467	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T234609 WP_000897305.1 NZ_CP091703:3269489-3269800 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T234609 NZ_LR130564:4372662-4373003 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT234609 WP_000356395.1 NZ_CP091703:3269801-3270091 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT234609 NZ_LR130564:c4372667-4372591 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7