Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 2044730..2045424 | Replicon | chromosome |
| Accession | NZ_CP091703 | ||
| Organism | Escherichia coli strain KOr021 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | Q0T7Q5 |
| Locus tag | L6L60_RS09665 | Protein ID | WP_001263491.1 |
| Coordinates | 2044730..2045128 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | L4JHF0 |
| Locus tag | L6L60_RS09670 | Protein ID | WP_000554755.1 |
| Coordinates | 2045131..2045424 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (2040559) | 2040559..2040639 | - | 81 | NuclAT_8 | - | - |
| - (2040559) | 2040559..2040639 | - | 81 | NuclAT_8 | - | - |
| - (2040559) | 2040559..2040639 | - | 81 | NuclAT_8 | - | - |
| - (2040559) | 2040559..2040639 | - | 81 | NuclAT_8 | - | - |
| L6L60_RS09635 (2039899) | 2039899..2041143 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| L6L60_RS09640 (2041235) | 2041235..2041693 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| L6L60_RS09645 (2041954) | 2041954..2043411 | + | 1458 | WP_001293009.1 | cytosol nonspecific dipeptidase | - |
| L6L60_RS09650 (2043468) | 2043468..2043820 | - | 353 | Protein_1894 | peptide chain release factor H | - |
| L6L60_RS09655 (2043816) | 2043816..2044022 | - | 207 | Protein_1895 | RtcB family protein | - |
| L6L60_RS09660 (2044268) | 2044268..2044720 | - | 453 | WP_019842500.1 | GNAT family N-acetyltransferase | - |
| L6L60_RS09665 (2044730) | 2044730..2045128 | - | 399 | WP_001263491.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| L6L60_RS09670 (2045131) | 2045131..2045424 | - | 294 | WP_000554755.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| L6L60_RS09675 (2045476) | 2045476..2046531 | - | 1056 | WP_001550655.1 | DNA polymerase IV | - |
| L6L60_RS09680 (2046602) | 2046602..2047387 | - | 786 | WP_019842510.1 | putative lateral flagellar export/assembly protein LafU | - |
| L6L60_RS09685 (2047359) | 2047359..2049071 | + | 1713 | Protein_1901 | flagellar biosynthesis protein FlhA | - |
| L6L60_RS09690 (2049176) | 2049176..2049454 | + | 279 | WP_000598760.1 | type II toxin-antitoxin system HicA family toxin | - |
| L6L60_RS09695 (2049447) | 2049447..2049803 | + | 357 | WP_001030484.1 | type II toxin-antitoxin system HicB family antitoxin | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 2034666..2045424 | 10758 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15457.86 Da Isoelectric Point: 8.0949
>T234606 WP_001263491.1 NZ_CP091703:c2045128-2044730 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T234606 NZ_LR130564:3757380-3757598 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11235.78 Da Isoelectric Point: 5.2170
>AT234606 WP_000554755.1 NZ_CP091703:c2045424-2045131 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT234606 NZ_LR130564:3756980-3757354 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4P7TPK7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829G9H5 |