Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4109173..4109900 Replicon chromosome
Accession NZ_CP091700
Organism Escherichia coli strain KFu019

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag L6L55_RS21130 Protein ID WP_000550189.1
Coordinates 4109586..4109900 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag L6L55_RS21125 Protein ID WP_000560255.1
Coordinates 4109173..4109589 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L55_RS21115 (4104533) 4104533..4106884 + 2352 Protein_3955 alpha-glucosidase -
L6L55_RS21120 (4107110) 4107110..4109128 + 2019 WP_023281493.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
L6L55_RS21125 (4109173) 4109173..4109589 - 417 WP_000560255.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
L6L55_RS21130 (4109586) 4109586..4109900 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
L6L55_RS21135 (4110184) 4110184..4111320 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
L6L55_RS21140 (4111405) 4111405..4111908 + 504 WP_001300832.1 M48 family metallopeptidase -
L6L55_RS21145 (4111985) 4111985..4112677 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
L6L55_RS21150 (4112756) 4112756..4113742 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T234589 WP_000550189.1 NZ_CP091700:c4109900-4109586 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T234589 NZ_LR130562:c4542188-4542015 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14951.41 Da        Isoelectric Point: 4.5577

>AT234589 WP_000560255.1 NZ_CP091700:c4109589-4109173 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNAPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT234589 NZ_LR130562:c4542512-4542216 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References