Detailed information of TA system TA234572

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2139496..2139867
• Replicon: chromosome
• Accession: NZ_CP091700
• Organism: Escherichia coli strain KFu019

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: L6L55_RS11325
• Protein ID: WP_001317028.1
• Coordinates: 2139673..2139867 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2139496..2139674 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L55_RS24955 (2135248)	2135248..2135421	+	174	WP_001296046.1	protein YnaL	-
L6L55_RS11300 (2135451)	2135451..2136824	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
L6L55_RS11305 (2136953)	2136953..2137888	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
L6L55_RS11310 (2137940)	2137940..2139175	-	1236	WP_000040852.1	site-specific integrase	-
L6L55_RS11315 (2139177)	2139177..2139392	-	216	WP_000079604.1	excisionase XisR	-
- (2139496)	2139496..2139674	+	179	NuclAT_0	-	Antitoxin
- (2139496)	2139496..2139674	+	179	NuclAT_0	-	Antitoxin
- (2139496)	2139496..2139674	+	179	NuclAT_0	-	Antitoxin
- (2139496)	2139496..2139674	+	179	NuclAT_0	-	Antitoxin
L6L55_RS11320 (2139471)	2139471..2139680	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
L6L55_RS11325 (2139673)	2139673..2139867	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
L6L55_RS11330 (2139924)	2139924..2140733	-	810	WP_000166319.1	recombination protein RecT	-
L6L55_RS11335 (2140726)	2140726..2143326	-	2601	WP_000105143.1	exodeoxyribonuclease VIII	-
L6L55_RS11340 (2143428)	2143428..2143703	-	276	WP_000632297.1	protein RacC	-
L6L55_RS11345 (2143778)	2143778..2143948	-	171	WP_001352098.1	YdaE family protein	-
L6L55_RS11350 (2143948)	2143948..2144169	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2130681..2160067	29386

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T234572 WP_001317028.1 NZ_CP091700:c2139867-2139673 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T234572 NZ_LR130562:1923614-1923865 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCACCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT234572 NZ_CP091700:2139496-2139674 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin