234564

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 975490..976184 Replicon chromosome
Accession NZ_CP091700
Organism Escherichia coli strain KFu019

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag L6L55_RS05515 Protein ID WP_001263493.1
Coordinates 975786..976184 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag L6L55_RS05510 Protein ID WP_000554757.1
Coordinates 975490..975783 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L55_RS05490 (971130) 971130..971627 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
L6L55_RS05495 (971843) 971843..973555 - 1713 Protein_887 flagellar biosynthesis protein FlhA -
L6L55_RS05500 (973527) 973527..974312 + 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
L6L55_RS05505 (974383) 974383..975438 + 1056 WP_085452615.1 DNA polymerase IV -
L6L55_RS05510 (975490) 975490..975783 + 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
L6L55_RS05515 (975786) 975786..976184 + 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
L6L55_RS05520 (976194) 976194..976646 + 453 WP_001059847.1 GNAT family N-acetyltransferase -
L6L55_RS05525 (976892) 976892..977095 + 204 Protein_893 RtcB family protein -
L6L55_RS05530 (977094) 977094..977615 + 522 Protein_894 peptide chain release factor H -
L6L55_RS05535 (977672) 977672..979129 - 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
L6L55_RS05540 (979390) 979390..979848 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (980444) 980444..980524 + 81 NuclAT_13 - -
- (980444) 980444..980524 + 81 NuclAT_13 - -
- (980444) 980444..980524 + 81 NuclAT_13 - -
- (980444) 980444..980524 + 81 NuclAT_13 - -
L6L55_RS05545 (979940) 979940..981184 + 1245 WP_000189541.1 esterase FrsA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - hcp1/tssD1 / tssA / gmhA/lpcA 941690..991256 49566


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T234564 WP_001263493.1 NZ_CP091700:975786-976184 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T234564 NZ_LR130562:219107-219214 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT234564 WP_000554757.1 NZ_CP091700:975490-975783 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT234564 NZ_LR130562:c219049-218995 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References