Detailed information of TA system TA234558

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 21799..22068
• Replicon: plasmid pKFu019_2
• Accession: NZ_CP091699
• Organism: Escherichia coli strain KFu019

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: L6L55_RS00665
• Protein ID: WP_001372321.1
• Coordinates: 21943..22068 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	21799..21864 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L55_RS00630	16838..17122	+	285	WP_072165043.1	hypothetical protein	-
L6L55_RS00635	17592..18119	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
L6L55_RS00640	18175..18408	+	234	WP_000006004.1	DUF905 domain-containing protein	-
L6L55_RS00645	18467..20425	+	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
L6L55_RS00650	20480..20914	+	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
L6L55_RS00655	20911..21673	+	763	Protein_34	plasmid SOS inhibition protein A	-
-	21642..21866	+	225	NuclAT_0	-	-
-	21642..21866	+	225	NuclAT_0	-	-
-	21642..21866	+	225	NuclAT_0	-	-
-	21642..21866	+	225	NuclAT_0	-	-
-	21799..21864	-	66	-	-	Antitoxin
L6L55_RS00660	21852..22001	+	150	Protein_35	plasmid maintenance protein Mok	-
L6L55_RS00665	21943..22068	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
L6L55_RS00670	22288..22518	+	231	WP_071587244.1	hypothetical protein	-
L6L55_RS00675	22516..22689	-	174	Protein_38	hypothetical protein	-
L6L55_RS00680	22987..23274	+	288	WP_000107537.1	hypothetical protein	-
L6L55_RS00685	23395..24216	+	822	WP_001234469.1	DUF932 domain-containing protein	-
L6L55_RS00690	24513..25160	-	648	WP_000614936.1	transglycosylase SLT domain-containing protein	-
L6L55_RS00695	25437..25820	+	384	WP_000124983.1	conjugal transfer relaxosome DNA-binding protein TraM	-
L6L55_RS00700	26011..26697	+	687	WP_240763344.1	PAS domain-containing protein	-
L6L55_RS00705	26791..27018	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T234558 WP_001372321.1 NZ_CP091699:21943-22068 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T234558 NZ_LR130556:109407-109823 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT234558 NZ_CP091699:c21864-21799 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin