Detailed information of TA system TA234554

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 9883..10147
• Replicon: plasmid pKFu019_1
• Accession: NZ_CP091698
• Organism: Escherichia coli strain KFu019

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: L6L55_RS00055
• Protein ID: WP_001387489.1
• Coordinates: 9883..10035 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	10087..10147 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L55_RS00025 (5149)	5149..7317	+	2169	WP_015508354.1	DotA/TraY family protein	-
L6L55_RS00030 (7388)	7388..8050	+	663	WP_012783935.1	plasmid IncI1-type surface exclusion protein ExcA	-
L6L55_RS00035 (8122)	8122..8331	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
L6L55_RS00040 (8723)	8723..8899	+	177	WP_001054900.1	hypothetical protein	-
L6L55_RS24895 (8964)	8964..9059	-	96	WP_000609148.1	DinQ-like type I toxin DqlB	-
L6L55_RS00050 (9560)	9560..9811	+	252	WP_001291964.1	hypothetical protein	-
L6L55_RS00055 (9883)	9883..10035	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
- (10087)	10087..10147	+	61	NuclAT_0	-	Antitoxin
- (10087)	10087..10147	+	61	NuclAT_0	-	Antitoxin
- (10087)	10087..10147	+	61	NuclAT_0	-	Antitoxin
- (10087)	10087..10147	+	61	NuclAT_0	-	Antitoxin
L6L55_RS00060 (10662)	10662..11441	+	780	WP_275450201.1	protein FinQ	-
- (11511)	11511..11568	+	58	NuclAT_1	-	-
- (11511)	11511..11568	+	58	NuclAT_1	-	-
- (11511)	11511..11568	+	58	NuclAT_1	-	-
- (11511)	11511..11568	+	58	NuclAT_1	-	-
L6L55_RS00065 (11748)	11748..12956	+	1209	WP_001703845.1	IncI1-type conjugal transfer protein TrbA	-
L6L55_RS00070 (12975)	12975..14045	+	1071	WP_012783932.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55	-	1..86740	86740

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T234554 WP_001387489.1 NZ_CP091698:c10035-9883 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T234554 NZ_LR130556:c5488-5213 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Nucleotide

• Length: 61 bp

>AT234554 NZ_CP091698:10087-10147 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin