Detailed information of TA system TA234550

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 63884..64137
• Replicon: plasmid pKMi006_2
• Accession: NZ_CP091693
• Organism: Escherichia coli strain KMi006

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: L6L65_RS24910
• Protein ID: WP_001312851.1
• Coordinates: 63884..64033 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	64078..64137 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L65_RS24875 (59348)	59348..60118	+	771	WP_000712166.1	molybdopterin-dependent oxidoreductase	-
L6L65_RS24880 (60148)	60148..60405	+	258	WP_167791825.1	hypothetical protein	-
L6L65_RS24885 (60555)	60555..60653	-	99	WP_137491101.1	transposase	-
L6L65_RS24890 (60846)	60846..61154	+	309	Protein_78	transposase	-
L6L65_RS24895 (62182)	62182..63039	-	858	WP_016240488.1	incFII family plasmid replication initiator RepA	-
L6L65_RS24900 (63032)	63032..63106	-	75	WP_001365705.1	RepA leader peptide Tap	-
L6L65_RS24905 (63351)	63351..63599	-	249	WP_000083839.1	replication regulatory protein RepA	-
L6L65_RS24910 (63884)	63884..64033	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (64078)	64078..64137	+	60	NuclAT_1	-	Antitoxin
- (64078)	64078..64137	+	60	NuclAT_1	-	Antitoxin
- (64078)	64078..64137	+	60	NuclAT_1	-	Antitoxin
- (64078)	64078..64137	+	60	NuclAT_1	-	Antitoxin
L6L65_RS24915 (64280)	64280..64753	-	474	WP_016240489.1	hypothetical protein	-
L6L65_RS24920 (64908)	64908..65498	-	591	WP_033807967.1	DUF2726 domain-containing protein	-
L6L65_RS24925 (65536)	65536..65745	-	210	WP_001298565.1	hemolysin expression modulator Hha	-
L6L65_RS24930 (65791)	65791..66252	-	462	WP_001233838.1	thermonuclease family protein	-
L6L65_RS24935 (66497)	66497..66709	-	213	WP_001309245.1	ANR family transcriptional regulator	-
L6L65_RS24940 (66838)	66838..67398	-	561	WP_000139321.1	fertility inhibition protein FinO	-
L6L65_RS24945 (67501)	67501..68361	-	861	WP_000704523.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B)	-	1..68938	68938
	-	flank	IS/Tn	-	-	60888..61154	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T234550 WP_001312851.1 NZ_CP091693:c64033-63884 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T234550 NZ_LR130555:c4325374-4325000 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT234550 NZ_CP091693:64078-64137 [Escherichia coli]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin