Detailed information of TA system TA234547

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 36194..36620
• Replicon: plasmid pKMi006_2
• Accession: NZ_CP091693
• Organism: Escherichia coli strain KMi006

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: L6L65_RS24710
• Protein ID: WP_001372321.1
• Coordinates: 36194..36319 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	36396..36620 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L65_RS24675 (31280)	31280..31507	-	228	WP_000589556.1	conjugal transfer relaxosome protein TraY	-
L6L65_RS24680 (31627)	31627..32274	-	648	WP_000332523.1	transcriptional regulator TraJ family protein	-
L6L65_RS24685 (32466)	32466..32849	-	384	WP_001151529.1	conjugal transfer relaxosome DNA-binding protein TraM	-
L6L65_RS24690 (33182)	33182..33772	+	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
L6L65_RS24695 (34069)	34069..34890	-	822	WP_001234445.1	DUF932 domain-containing protein	-
L6L65_RS24700 (35001)	35001..35297	-	297	WP_001272251.1	hypothetical protein	-
L6L65_RS24705 (35597)	35597..35893	+	297	Protein_41	hypothetical protein	-
L6L65_RS24710 (36194)	36194..36319	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
L6L65_RS24715 (36261)	36261..36410	-	150	Protein_43	plasmid maintenance protein Mok	-
L6L65_RS24720 (36432)	36432..36611	+	180	WP_001309233.1	hypothetical protein	-
- (36396)	36396..36620	-	225	NuclAT_0	-	Antitoxin
- (36396)	36396..36620	-	225	NuclAT_0	-	Antitoxin
- (36396)	36396..36620	-	225	NuclAT_0	-	Antitoxin
- (36396)	36396..36620	-	225	NuclAT_0	-	Antitoxin
L6L65_RS24725 (36589)	36589..37351	-	763	Protein_45	plasmid SOS inhibition protein A	-
L6L65_RS24730 (37348)	37348..37782	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
L6L65_RS24735 (37851)	37851..39815	-	1965	WP_117389470.1	ParB/RepB/Spo0J family partition protein	-
L6L65_RS24740 (39876)	39876..40109	-	234	WP_000006003.1	DUF905 family protein	-
L6L65_RS24745 (40167)	40167..40694	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
L6L65_RS24750 (40996)	40996..41457	+	462	Protein_50	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B)	-	1..68938	68938

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T234547 WP_001372321.1 NZ_CP091693:c36319-36194 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T234547 NZ_LR130555:c3949496-3949098 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT234547 NZ_CP091693:c36620-36396 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin