Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4234852..4235651 Replicon chromosome
Accession NZ_CP091691
Organism Escherichia coli strain KMi006

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag L6L65_RS20545 Protein ID WP_000347275.1
Coordinates 4234852..4235316 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag L6L65_RS20550 Protein ID WP_001307405.1
Coordinates 4235316..4235651 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L65_RS20515 (4229853) 4229853..4230287 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
L6L65_RS20520 (4230305) 4230305..4231183 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
L6L65_RS20525 (4231173) 4231173..4231952 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
L6L65_RS20530 (4231963) 4231963..4232436 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
L6L65_RS20535 (4232459) 4232459..4233739 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
L6L65_RS20540 (4233988) 4233988..4234797 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
L6L65_RS20545 (4234852) 4234852..4235316 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
L6L65_RS20550 (4235316) 4235316..4235651 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
L6L65_RS20555 (4235800) 4235800..4237371 - 1572 WP_001273752.1 galactarate dehydratase -
L6L65_RS20560 (4237746) 4237746..4239080 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
L6L65_RS20565 (4239096) 4239096..4239866 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T234539 WP_000347275.1 NZ_CP091691:c4235316-4234852 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T234539 NZ_LR130555:2260498-2260601 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT234539 WP_001307405.1 NZ_CP091691:c4235651-4235316 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT234539 NZ_LR130555:c2260639-2260368 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References