Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2585345..2586039 Replicon chromosome
Accession NZ_CP091691
Organism Escherichia coli strain KMi006

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag L6L65_RS12645 Protein ID WP_001263489.1
Coordinates 2585345..2585743 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag L6L65_RS12650 Protein ID WP_000554758.1
Coordinates 2585746..2586039 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (2580933) 2580933..2581013 - 81 NuclAT_13 - -
- (2580933) 2580933..2581013 - 81 NuclAT_13 - -
- (2580933) 2580933..2581013 - 81 NuclAT_13 - -
- (2580933) 2580933..2581013 - 81 NuclAT_13 - -
L6L65_RS12620 (2581609) 2581609..2582067 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
L6L65_RS12625 (2582328) 2582328..2583785 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
L6L65_RS12630 (2583842) 2583842..2584363 - 522 Protein_2489 peptide chain release factor H -
L6L65_RS12635 (2584359) 2584359..2584565 - 207 Protein_2490 RtcB family protein -
L6L65_RS12640 (2584883) 2584883..2585335 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
L6L65_RS12645 (2585345) 2585345..2585743 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
L6L65_RS12650 (2585746) 2585746..2586039 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
L6L65_RS12655 (2586091) 2586091..2587146 - 1056 WP_001226164.1 DNA polymerase IV -
L6L65_RS12660 (2587217) 2587217..2588002 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
L6L65_RS12665 (2587974) 2587974..2589686 + 1713 Protein_2496 flagellar biosynthesis protein FlhA -
L6L65_RS12670 (2589910) 2589910..2590407 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T234534 WP_001263489.1 NZ_CP091691:c2585743-2585345 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T234534 NZ_LR130555:243440-243547 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT234534 WP_000554758.1 NZ_CP091691:c2586039-2585746 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT234534 NZ_LR130555:c243383-243328 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References