Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 652821..653323 Replicon chromosome
Accession NZ_CP091691
Organism Escherichia coli strain KMi006

Toxin (Protein)


Gene name yoeB Uniprot ID A0A7I6H4L3
Locus tag L6L65_RS03045 Protein ID WP_000767831.1
Coordinates 653069..653323 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag L6L65_RS03040 Protein ID WP_001259255.1
Coordinates 652821..653072 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L65_RS03015 (647998) 647998..649065 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
L6L65_RS03020 (649065) 649065..650135 - 1071 WP_000108945.1 histidinol-phosphate transaminase -
L6L65_RS03025 (650132) 650132..651436 - 1305 WP_001446927.1 histidinol dehydrogenase -
L6L65_RS03030 (651442) 651442..652341 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
L6L65_RS03035 (652487) 652487..652537 - 51 WP_001364200.1 his operon leader peptide -
L6L65_RS03040 (652821) 652821..653072 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
L6L65_RS03045 (653069) 653069..653323 + 255 WP_000767831.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
L6L65_RS03050 (653406) 653406..654230 + 825 WP_000754737.1 SDR family oxidoreductase -
L6L65_RS03055 (654276) 654276..655205 + 930 WP_024195433.1 LysR substrate-binding domain-containing protein -
L6L65_RS03060 (655420) 655420..655482 + 63 WP_010723108.1 membrane protein YoeI -
L6L65_RS03065 (655472) 655472..656830 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
L6L65_RS03070 (657009) 657009..658067 + 1059 WP_000492321.1 thiosulfate utilization transporter TsuA/YeeE -
L6L65_RS03075 (658081) 658081..658308 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10231.67 Da        Isoelectric Point: 8.8202

>T234521 WP_000767831.1 NZ_CP091691:653069-653323 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTYDSLLIAAC
RYHY

Download         Length: 255 bp

>T234521 NZ_LR130552:3517479-3517697 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT234521 WP_001259255.1 NZ_CP091691:652821-653072 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT234521 NZ_LR130552:3517079-3517453 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7I6H4L3

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References