Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2270640..2271202 Replicon chromosome
Accession NZ_CP091682
Organism Escherichia coli strain KKo003

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag L6L54_RS11155 Protein ID WP_000605675.1
Coordinates 2270924..2271202 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag L6L54_RS11150 Protein ID WP_000781370.1
Coordinates 2270640..2270924 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L54_RS11135 (2265821) 2265821..2268868 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
L6L54_RS11140 (2268881) 2268881..2269765 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
L6L54_RS11145 (2269758) 2269758..2270411 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
L6L54_RS11150 (2270640) 2270640..2270924 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
L6L54_RS11155 (2270924) 2270924..2271202 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L6L54_RS11160 (2271388) 2271388..2272398 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
L6L54_RS11165 (2272532) 2272532..2274229 - 1698 WP_000433460.1 malate dehydrogenase -
L6L54_RS11170 (2274386) 2274386..2274523 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
L6L54_RS11175 (2274625) 2274625..2274840 - 216 WP_000495766.1 biofilm-dependent modulation protein -
L6L54_RS11180 (2275185) 2275185..2275616 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T234501 WP_000605675.1 NZ_CP091682:c2271202-2270924 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T234501 NZ_LR130548:c4736245-4735712 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT234501 WP_000781370.1 NZ_CP091682:c2270924-2270640 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT234501 NZ_LR130548:c4736522-4736256 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References