Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1002267..1002961 Replicon chromosome
Accession NZ_CP091682
Organism Escherichia coli strain KKo003

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag L6L54_RS04820 Protein ID WP_001263500.1
Coordinates 1002563..1002961 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag L6L54_RS04815 Protein ID WP_000554758.1
Coordinates 1002267..1002560 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L54_RS04790 (998084) 998084..998551 + 468 WP_000725261.1 flagellar basal body-associated FliL family protein -
L6L54_RS04795 (998571) 998571..999287 + 717 WP_000938731.1 FliA/WhiG family RNA polymerase sigma factor -
L6L54_RS04800 (999300) 999300..1000163 + 864 WP_001169518.1 flagellar motor stator protein MotA -
L6L54_RS04805 (1000166) 1000166..1001089 + 924 WP_001532973.1 putative lateral flagellar export/assembly protein LafU -
L6L54_RS04810 (1001160) 1001160..1002215 + 1056 WP_001226168.1 DNA polymerase IV -
L6L54_RS04815 (1002267) 1002267..1002560 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
L6L54_RS04820 (1002563) 1002563..1002961 + 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
L6L54_RS04825 (1002971) 1002971..1003423 + 453 WP_001059895.1 GNAT family N-acetyltransferase -
L6L54_RS04830 (1003613) 1003613..1004752 + 1140 WP_000521561.1 RNA ligase RtcB family protein -
L6L54_RS04835 (1004749) 1004749..1005363 + 615 WP_000602124.1 peptide chain release factor H -
L6L54_RS04840 (1005420) 1005420..1006877 - 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
L6L54_RS04845 (1007138) 1007138..1007596 + 459 WP_001291991.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T234498 WP_001263500.1 NZ_CP091682:1002563-1002961 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T234498 NZ_LR130548:2013229-2013331 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT234498 WP_000554758.1 NZ_CP091682:1002267-1002560 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT234498 NZ_LR130548:c2013338-2013193 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References