Detailed information of TA system TA234491

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 611065..611477
• Replicon: chromosome
• Accession: NZ_CP091682
• Organism: Escherichia coli strain KKo003

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: L6L54_RS02945
• Protein ID: WP_000132614.1
• Coordinates: 611065..611406 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	611401..611477 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L54_RS02920 (606134)	606134..607414	-	1281	WP_001338077.1	DUF445 domain-containing protein	-
L6L54_RS02925 (607599)	607599..608519	+	921	WP_001513532.1	Rpn family recombination-promoting nuclease/putative transposase	-
L6L54_RS02930 (608747)	608747..608827	+	81	WP_020233658.1	hypothetical protein	-
L6L54_RS02935 (608925)	608925..609801	+	877	Protein_572	DUF262 domain-containing protein	-
L6L54_RS02940 (609855)	609855..611018	+	1164	WP_224153787.1	DUF1524 domain-containing protein	-
L6L54_RS02945 (611065)	611065..611406	-	342	WP_000132614.1	endoribonuclease SymE	Toxin
- (611401)	611401..611477	+	77	NuclAT_8	-	Antitoxin
- (611401)	611401..611477	+	77	NuclAT_8	-	Antitoxin
- (611401)	611401..611477	+	77	NuclAT_8	-	Antitoxin
- (611401)	611401..611477	+	77	NuclAT_8	-	Antitoxin
- (611401)	611401..611477	+	77	NuclAT_9	-	Antitoxin
- (611401)	611401..611477	+	77	NuclAT_9	-	Antitoxin
- (611401)	611401..611477	+	77	NuclAT_9	-	Antitoxin
- (611401)	611401..611477	+	77	NuclAT_9	-	Antitoxin
L6L54_RS02950 (611627)	611627..613396	-	1770	WP_001513535.1	restriction endonuclease subunit S	-
L6L54_RS02955 (613396)	613396..614865	-	1470	WP_001387312.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T234491 WP_000132614.1 NZ_CP091682:c611406-611065 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T234491 NZ_LR130546:c98910-98494 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT234491 NZ_CP091682:611401-611477 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin