Detailed information of TA system TA234480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3798788..3799515
• Replicon: chromosome
• Accession: NZ_CP091677
• Organism: Escherichia coli strain KFu022

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1EX98
• Locus tag: L6L66_RS18490
• Protein ID: WP_000550192.1
• Coordinates: 3799201..3799515 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L6L66_RS18485
• Protein ID: WP_000560266.1
• Coordinates: 3798788..3799204 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L66_RS18475 (3793948)	3793948..3796299	+	2352	WP_000695486.1	alpha-glucosidase	-
L6L66_RS18480 (3796725)	3796725..3798743	+	2019	WP_000121433.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
L6L66_RS18485 (3798788)	3798788..3799204	-	417	WP_000560266.1	type II toxin-antitoxin system antitoxin HigA	Antitoxin
L6L66_RS18490 (3799201)	3799201..3799515	-	315	WP_000550192.1	type II toxin-antitoxin system toxin HigB	Toxin
L6L66_RS18495 (3799799)	3799799..3800935	-	1137	WP_000018695.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
L6L66_RS18500 (3801020)	3801020..3801523	+	504	WP_001295542.1	M48 family metallopeptidase	-
L6L66_RS18505 (3801600)	3801600..3802283	+	684	WP_001183046.1	vancomycin high temperature exclusion protein	-
L6L66_RS18510 (3802362)	3802362..3803348	+	987	WP_032238269.1	Gfo/Idh/MocA family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-135)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12063.08 Da
• Isoelectric Point: 10.0409

>T234480 WP_000550192.1 NZ_CP091677:c3799515-3799201 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTPKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T234480 NZ_CP091677:c3799515-3799201 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCCTAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14995.42 Da
• Isoelectric Point: 4.4547

>AT234480 WP_000560266.1 NZ_CP091677:c3799204-3798788 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT234480 NZ_CP091677:c3799204-3798788 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: S1EX98