Detailed information of TA system TA023446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 85685..86293
• Replicon: chromosome
• Accession: NC_011761
• Organism: Acidithiobacillus ferrooxidans ATCC 23270

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2W1K1W3
• Locus tag: AFE_RS00435
• Protein ID: WP_012535782.1
• Coordinates: 85685..85960 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: AFE_RS00440
• Protein ID: WP_009566298.1
• Coordinates: 85976..86293 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFE_RS00395	81463..82503	+	1041	WP_012535779.1	glycosyltransferase family 4 protein	-
AFE_RS00400	82571..83497	+	927	WP_012535780.1	glycosyltransferase family 2 protein	-
AFE_RS00405	83502..83858	+	357	Protein_81	glycosyltransferase	-
AFE_RS00420	84598..84819	+	222	WP_009566302.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
AFE_RS00425	84819..85232	+	414	WP_009566301.1	type II toxin-antitoxin system VapC family toxin	-
AFE_RS00430	85229..85519	+	291	WP_009566300.1	hypothetical protein	-
AFE_RS00435	85685..85960	+	276	WP_012535782.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AFE_RS00440	85976..86293	+	318	WP_009566298.1	HigA family addiction module antidote protein	Antitoxin
AFE_RS00445	86308..86487	+	180	WP_009566297.1	HNH endonuclease	-
AFE_RS00450	86907..88070	+	1164	WP_009566294.1	Fic family protein	-
AFE_RS16560	88367..88633	-	267	WP_196491178.1	hypothetical protein	-
AFE_RS00460	88630..88872	-	243	WP_012535785.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
AFE_RS00465	88956..89129	-	174	WP_009562770.1	PCP reductase family protein	-
AFE_RS00470	89240..89668	-	429	WP_009562768.1	hypothetical protein	-
AFE_RS00475	89671..90102	-	432	WP_009562767.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-91)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-69)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10433.84 Da
• Isoelectric Point: 7.9070

>T23446 WP_012535782.1 NC_011761:85685-85960 [Acidithiobacillus ferrooxidans ATCC 23270]
MIRSFVHKGLKQVFETGQSAKVGADLVTRCKRRLDAINAATSPEELNVPGFDFHALEGKPKRYTVHVNGPYCITFEWREG
DAWRIDLENYH

Nucleotide

• Length: 276 bp

>T23446 NC_011761:85685-85960 [Acidithiobacillus ferrooxidans ATCC 23270]
TTGATACGAAGTTTTGTGCATAAGGGCCTCAAGCAGGTTTTCGAAACAGGTCAATCGGCGAAAGTCGGTGCGGATTTGGT
AACCCGCTGCAAGCGCAGGCTCGATGCCATAAACGCTGCGACATCCCCGGAGGAGTTGAACGTGCCGGGTTTCGACTTCC
ATGCTTTGGAGGGGAAACCGAAGCGGTACACGGTTCATGTGAATGGACCCTATTGCATCACCTTCGAGTGGCGTGAAGGA
GATGCCTGGCGCATTGATCTGGAGAATTACCATTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11756.54 Da
• Isoelectric Point: 5.6631

>AT23446 WP_009566298.1 NC_011761:85976-86293 [Acidithiobacillus ferrooxidans ATCC 23270]
MDEMTVEQVDRIPTHPGEILREEVLPALDISVAEFARGLRVSRQTVHGILSERKGITPEMALRIGKYLGNGPGIWLRMQQ
AYDLTVAERRLAEELAAIHKPEQAA

Nucleotide

• Length: 318 bp

>AT23446 NC_011761:85976-86293 [Acidithiobacillus ferrooxidans ATCC 23270]
ATGGACGAAATGACCGTTGAGCAAGTGGATCGGATACCGACGCATCCTGGCGAGATATTGCGGGAAGAGGTACTGCCCGC
ACTGGATATCTCTGTAGCAGAGTTTGCCCGTGGCCTGCGGGTCTCGCGCCAGACCGTTCACGGCATCCTGTCTGAGCGCA
AGGGCATCACCCCGGAGATGGCCTTGCGTATCGGCAAATATCTGGGTAACGGGCCGGGGATATGGCTACGCATGCAGCAG
GCTTACGATTTGACCGTTGCAGAACGTCGGTTGGCCGAAGAACTGGCGGCCATTCACAAGCCCGAGCAGGCCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A2W1K1W3