Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3981673..3982472 Replicon chromosome
Accession NZ_CP091673
Organism Escherichia coli strain KKo009

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag L6L68_RS20300 Protein ID WP_000347251.1
Coordinates 3982008..3982472 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag L6L68_RS20295 Protein ID WP_001296435.1
Coordinates 3981673..3982008 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L68_RS20280 (3977458) 3977458..3978228 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
L6L68_RS20285 (3978244) 3978244..3979578 - 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
L6L68_RS20290 (3979953) 3979953..3981524 + 1572 WP_001273941.1 galactarate dehydratase -
L6L68_RS20295 (3981673) 3981673..3982008 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
L6L68_RS20300 (3982008) 3982008..3982472 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
L6L68_RS20305 (3982527) 3982527..3983336 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
L6L68_RS20310 (3983585) 3983585..3984865 + 1281 WP_000681938.1 tagatose-bisphosphate aldolase subunit KbaZ -
L6L68_RS20315 (3984888) 3984888..3985361 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
L6L68_RS20320 (3985372) 3985372..3986151 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
L6L68_RS20325 (3986141) 3986141..3987019 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
L6L68_RS20330 (3987037) 3987037..3987471 + 435 WP_000948823.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T234443 WP_000347251.1 NZ_CP091673:3982008-3982472 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T234443 NZ_LR130543:315517-315885 [Klebsiella variicola]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGACGTTGCCGG
TATGCCCGACCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCACCTGCTGGTGATTTCTCGCGGCCATATTTTCAATGATGGAAATAAGCGTACGGCACTGTTT
ATTACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCGGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT234443 WP_001296435.1 NZ_CP091673:3981673-3982008 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT234443 NZ_LR130543:315299-315520 [Klebsiella variicola]
ATGAGAACGGTTAACTATAGCGAAGCCCGACAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCGGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATTATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References