Detailed information of TA system TA234415

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 104312..104738
• Replicon: plasmid pKOr019_1
• Accession: NZ_CP091666
• Organism: Escherichia coli strain KOr019

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: L6L58_RS25465
• Protein ID: WP_096937776.1
• Coordinates: 104312..104437 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	104514..104738 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L58_RS25430 (99485)	99485..99886	-	402	WP_001369361.1	conjugal transfer relaxosome DNA-bindin protein TraY	-
L6L58_RS25435 (99979)	99979..100668	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
L6L58_RS25440 (100855)	100855..101238	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
L6L58_RS25445 (101559)	101559..102161	+	603	WP_077250849.1	transglycosylase SLT domain-containing protein	-
L6L58_RS25450 (102458)	102458..103279	-	822	WP_001234487.1	DUF932 domain-containing protein	-
L6L58_RS25455 (103390)	103390..103686	-	297	WP_001272245.1	hypothetical protein	-
L6L58_RS25460 (103738)	103738..104011	+	274	Protein_105	hypothetical protein	-
L6L58_RS25465 (104312)	104312..104437	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
L6L58_RS25470 (104379)	104379..104528	-	150	Protein_107	plasmid maintenance protein Mok	-
- (104514)	104514..104738	-	225	NuclAT_0	-	Antitoxin
- (104514)	104514..104738	-	225	NuclAT_0	-	Antitoxin
- (104514)	104514..104738	-	225	NuclAT_0	-	Antitoxin
- (104514)	104514..104738	-	225	NuclAT_0	-	Antitoxin
L6L58_RS25475 (104707)	104707..105469	-	763	Protein_108	plasmid SOS inhibition protein A	-
L6L58_RS25480 (105466)	105466..105900	-	435	WP_000845962.1	conjugation system SOS inhibitor PsiB	-
L6L58_RS25485 (105969)	105969..107933	-	1965	WP_001593049.1	ParB/RepB/Spo0J family partition protein	-
L6L58_RS25490 (107992)	107992..108225	-	234	WP_000005985.1	DUF905 family protein	-
L6L58_RS25495 (108283)	108283..108822	-	540	WP_001593051.1	single-stranded DNA-binding protein	-
L6L58_RS25500 (108848)	108848..109054	-	207	WP_000547969.1	hypothetical protein	-
L6L58_RS25505 (109124)	109124..109204	+	81	Protein_114	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..158848	158848
	-	flank	IS/Tn	-	-	109375..110346	971

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T234415 WP_096937776.1 NZ_CP091666:c104437-104312 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T234415 NZ_LR130539:346346-346714 [Klebsiella variicola]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGACCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTATGACAGACGTGT
GGCGACTCGCTGCGATGCACCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCCGACTTCGTCGGCATGACCGTCGAGGCTGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT234415 NZ_CP091666:c104738-104514 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGATTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin