Detailed information of TA system TA234380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4927450..4928052
• Replicon: chromosome
• Accession: NZ_CP091664
• Organism: Escherichia coli strain KKa019

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: L6L56_RS23830
• Protein ID: WP_000897305.1
• Coordinates: 4927741..4928052 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L6L56_RS23825
• Protein ID: WP_000356395.1
• Coordinates: 4927450..4927740 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L56_RS23790 (4923074)	4923074..4923976	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
L6L56_RS23795 (4923973)	4923973..4924608	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
L6L56_RS23800 (4924605)	4924605..4925534	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
L6L56_RS23805 (4925716)	4925716..4925958	-	243	WP_001309881.1	CopG family transcriptional regulator	-
L6L56_RS23810 (4926177)	4926177..4926395	-	219	WP_001295676.1	CopG family transcriptional regulator	-
L6L56_RS23815 (4926814)	4926814..4927092	-	279	WP_001315112.1	hypothetical protein	-
L6L56_RS23820 (4927144)	4927144..4927365	-	222	WP_001550354.1	hypothetical protein	-
L6L56_RS23825 (4927450)	4927450..4927740	-	291	WP_000356395.1	NadS family protein	Antitoxin
L6L56_RS23830 (4927741)	4927741..4928052	-	312	WP_000897305.1	hypothetical protein	Toxin
L6L56_RS23835 (4928281)	4928281..4929189	+	909	WP_001550353.1	alpha/beta hydrolase	-
L6L56_RS23840 (4929357)	4929357..4930271	-	915	WP_109553727.1	transposase	-
L6L56_RS23845 (4930284)	4930284..4931171	-	888	Protein_4672	hypothetical protein	-
L6L56_RS23850 (4931587)	4931587..4932528	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
L6L56_RS23855 (4932573)	4932573..4933010	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T234380 WP_000897305.1 NZ_CP091664:c4928052-4927741 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T234380 NZ_CP091664:c4928052-4927741 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT234380 WP_000356395.1 NZ_CP091664:c4927740-4927450 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT234380 NZ_CP091664:c4927740-4927450 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7