Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 4030713..4031407 | Replicon | chromosome |
| Accession | NZ_CP091664 | ||
| Organism | Escherichia coli strain KKa019 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | Q0T7Q5 |
| Locus tag | L6L56_RS19600 | Protein ID | WP_001263491.1 |
| Coordinates | 4030713..4031111 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | L4JHF0 |
| Locus tag | L6L56_RS19605 | Protein ID | WP_000554755.1 |
| Coordinates | 4031114..4031407 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (4026542) | 4026542..4026622 | - | 81 | NuclAT_9 | - | - |
| - (4026542) | 4026542..4026622 | - | 81 | NuclAT_9 | - | - |
| - (4026542) | 4026542..4026622 | - | 81 | NuclAT_9 | - | - |
| - (4026542) | 4026542..4026622 | - | 81 | NuclAT_9 | - | - |
| L6L56_RS19570 (4025882) | 4025882..4027126 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| L6L56_RS19575 (4027218) | 4027218..4027676 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| L6L56_RS19580 (4027937) | 4027937..4029394 | + | 1458 | WP_001293009.1 | cytosol nonspecific dipeptidase | - |
| L6L56_RS19585 (4029451) | 4029451..4029803 | - | 353 | Protein_3847 | peptide chain release factor H | - |
| L6L56_RS19590 (4029799) | 4029799..4030005 | - | 207 | Protein_3848 | RtcB family protein | - |
| L6L56_RS19595 (4030251) | 4030251..4030703 | - | 453 | WP_019842500.1 | GNAT family N-acetyltransferase | - |
| L6L56_RS19600 (4030713) | 4030713..4031111 | - | 399 | WP_001263491.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| L6L56_RS19605 (4031114) | 4031114..4031407 | - | 294 | WP_000554755.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| L6L56_RS19610 (4031459) | 4031459..4032514 | - | 1056 | WP_001550655.1 | DNA polymerase IV | - |
| L6L56_RS19615 (4032585) | 4032585..4033370 | - | 786 | WP_019842510.1 | putative lateral flagellar export/assembly protein LafU | - |
| L6L56_RS19620 (4033342) | 4033342..4035054 | + | 1713 | Protein_3854 | flagellar biosynthesis protein FlhA | - |
| L6L56_RS19625 (4035159) | 4035159..4035437 | + | 279 | WP_000598760.1 | type II toxin-antitoxin system HicA family toxin | - |
| L6L56_RS19630 (4035430) | 4035430..4035786 | + | 357 | WP_001030484.1 | type II toxin-antitoxin system HicB family antitoxin | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 4020649..4031407 | 10758 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15457.86 Da Isoelectric Point: 8.0949
>T234376 WP_001263491.1 NZ_CP091664:c4031111-4030713 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T234376 NZ_LR130533:c3463238-3462957 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11235.78 Da Isoelectric Point: 5.2170
>AT234376 WP_000554755.1 NZ_CP091664:c4031407-4031114 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT234376 NZ_LR130533:c3463462-3463235 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4P7TPK7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829G9H5 |