Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1994167..1994669 Replicon chromosome
Accession NZ_CP091664
Organism Escherichia coli strain KKa019

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag L6L56_RS09515 Protein ID WP_000767829.1
Coordinates 1994415..1994669 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag L6L56_RS09510 Protein ID WP_001259255.1
Coordinates 1994167..1994418 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L56_RS09485 (1989345) 1989345..1990412 - 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
L6L56_RS09490 (1990412) 1990412..1991482 - 1071 WP_000108937.1 histidinol-phosphate transaminase -
L6L56_RS09495 (1991479) 1991479..1992783 - 1305 WP_021523144.1 histidinol dehydrogenase -
L6L56_RS09500 (1992789) 1992789..1993688 - 900 WP_021523143.1 ATP phosphoribosyltransferase -
L6L56_RS09505 (1993834) 1993834..1993884 - 51 WP_001364200.1 his operon leader peptide -
L6L56_RS09510 (1994167) 1994167..1994418 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
L6L56_RS09515 (1994415) 1994415..1994669 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
L6L56_RS09520 (1994752) 1994752..1995576 + 825 WP_000754758.1 SDR family oxidoreductase -
L6L56_RS09525 (1995622) 1995622..1996551 + 930 WP_023154044.1 LysR substrate-binding domain-containing protein -
L6L56_RS09530 (1996766) 1996766..1996828 + 63 WP_010723108.1 membrane protein YoeI -
L6L56_RS09535 (1996818) 1996818..1998176 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
L6L56_RS09540 (1998355) 1998355..1999413 + 1059 WP_000492324.1 thiosulfate utilization transporter TsuA/YeeE -
L6L56_RS09545 (1999427) 1999427..1999654 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T234361 WP_000767829.1 NZ_CP091664:1994415-1994669 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T234361 NZ_LR130532:c2801879-2801776 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT234361 WP_001259255.1 NZ_CP091664:1994167-1994418 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT234361 NZ_LR130532:2801738-2802009 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References