Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 628585..629384 Replicon chromosome
Accession NZ_CP091664
Organism Escherichia coli strain KKa019

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag L6L56_RS03020 Protein ID WP_000347270.1
Coordinates 628585..629049 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag L6L56_RS03025 Protein ID WP_001551693.1
Coordinates 629049..629384 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L56_RS02990 (623586) 623586..624020 - 435 WP_000948837.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
L6L56_RS02995 (624038) 624038..624916 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
L6L56_RS03000 (624906) 624906..625685 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
L6L56_RS03005 (625696) 625696..626169 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
L6L56_RS03010 (626192) 626192..627472 - 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
L6L56_RS03015 (627721) 627721..628530 + 810 WP_106106567.1 aga operon transcriptional regulator AgaR -
L6L56_RS03020 (628585) 628585..629049 - 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
L6L56_RS03025 (629049) 629049..629384 - 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
L6L56_RS03030 (629533) 629533..631104 - 1572 WP_001273738.1 galactarate dehydratase -
L6L56_RS03035 (631479) 631479..632813 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
L6L56_RS03040 (632829) 632829..633599 + 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T234355 WP_000347270.1 NZ_CP091664:c629049-628585 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

>T234355 NZ_LR130532:c1008711-1008433 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT234355 WP_001551693.1 NZ_CP091664:c629384-629049 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT234355 NZ_LR130532:c1008974-1008714 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References