Detailed information of TA system TA023435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3789846..3790645
• Replicon: chromosome
• Accession: NC_011750
• Organism: Escherichia coli IAI39

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: ECIAI39_RS18235
• Protein ID: WP_000347273.1
• Coordinates: 3790181..3790645 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A210GLR0
• Locus tag: ECIAI39_RS18230
• Protein ID: WP_001571956.1
• Coordinates: 3789846..3790181 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECIAI39_RS18215	3785631..3786401	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
ECIAI39_RS18220	3786417..3787751	-	1335	WP_000599656.1	galactarate/glucarate/glycerate transporter GarP	-
ECIAI39_RS18225	3788126..3789697	+	1572	WP_001273756.1	galactarate dehydratase	-
ECIAI39_RS18230	3789846..3790181	+	336	WP_001571956.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
ECIAI39_RS18235	3790181..3790645	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
ECIAI39_RS18240	3790700..3791509	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
ECIAI39_RS18245	3791758..3793038	+	1281	WP_000681956.1	tagatose-bisphosphate aldolase subunit KbaZ	-
ECIAI39_RS18250	3793061..3793534	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
ECIAI39_RS18255	3793545..3794324	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
ECIAI39_RS18260	3794314..3795192	+	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
ECIAI39_RS18265	3795210..3795644	+	435	WP_000948834.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3781205..3790645	9440

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T23435 WP_000347273.1 NC_011750:3790181-3790645 [Escherichia coli IAI39]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T23435 NC_011750:3790181-3790645 [Escherichia coli IAI39]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12328.96 Da
• Isoelectric Point: 4.6647

>AT23435 WP_001571956.1 NC_011750:3789846-3790181 [Escherichia coli IAI39]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQITRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT23435 NC_011750:3789846-3790181 [Escherichia coli IAI39]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAATAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCAGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: A0A210GLR0