Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3592827..3593368 Replicon chromosome
Accession NZ_CP091662
Organism Escherichia coli strain KFu023

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag L6L71_RS17360 Protein ID WP_000615983.1
Coordinates 3593090..3593368 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag L6L71_RS17355 Protein ID WP_000729703.1
Coordinates 3592827..3593087 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L71_RS17335 (3588502) 3588502..3589287 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
L6L71_RS17340 (3589259) 3589259..3590971 + 1713 Protein_3405 flagellar biosynthesis protein FlhA -
L6L71_RS17345 (3591195) 3591195..3591692 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
L6L71_RS17350 (3591868) 3591868..3592617 - 750 WP_000056849.1 C40 family peptidase -
L6L71_RS17355 (3592827) 3592827..3593087 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
L6L71_RS17360 (3593090) 3593090..3593368 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
L6L71_RS17365 (3593524) 3593524..3594264 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
L6L71_RS17370 (3594235) 3594235..3595002 - 768 WP_000333380.1 class II glutamine amidotransferase -
L6L71_RS17375 (3595208) 3595208..3595786 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fdeC / ykgK/ecpR / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE / gmhA/lpcA 3539962..3601910 61948


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T234349 WP_000615983.1 NZ_CP091662:3593090-3593368 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T234349 NZ_LR130531:c3893462-3893181 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAAGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT234349 WP_000729703.1 NZ_CP091662:3592827-3593087 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT234349 NZ_LR130531:c3893686-3893459 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References