Detailed information of TA system TA234348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3586630..3587324
• Replicon: chromosome
• Accession: NZ_CP091662
• Organism: Escherichia coli strain KFu023

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: L6L71_RS17320
• Protein ID: WP_001263489.1
• Coordinates: 3586630..3587028 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: L6L71_RS17325
• Protein ID: WP_000554758.1
• Coordinates: 3587031..3587324 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3582218)	3582218..3582298	-	81	NuclAT_12	-	-
- (3582218)	3582218..3582298	-	81	NuclAT_12	-	-
- (3582218)	3582218..3582298	-	81	NuclAT_12	-	-
- (3582218)	3582218..3582298	-	81	NuclAT_12	-	-
L6L71_RS17295 (3582894)	3582894..3583352	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
L6L71_RS17300 (3583613)	3583613..3585070	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
L6L71_RS17305 (3585127)	3585127..3585648	-	522	Protein_3398	peptide chain release factor H	-
L6L71_RS17310 (3585644)	3585644..3585850	-	207	Protein_3399	RtcB family protein	-
L6L71_RS17315 (3586168)	3586168..3586620	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
L6L71_RS17320 (3586630)	3586630..3587028	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
L6L71_RS17325 (3587031)	3587031..3587324	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
L6L71_RS17330 (3587376)	3587376..3588431	-	1056	WP_001226164.1	DNA polymerase IV	-
L6L71_RS17335 (3588502)	3588502..3589287	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
L6L71_RS17340 (3589259)	3589259..3590971	+	1713	Protein_3405	flagellar biosynthesis protein FlhA	-
L6L71_RS17345 (3591195)	3591195..3591692	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE / gmhA/lpcA	3539962..3601910	61948

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T234348 WP_001263489.1 NZ_CP091662:c3587028-3586630 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T234348 NZ_LR130531:c2709520-2709242 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT234348 WP_000554758.1 NZ_CP091662:c3587324-3587031 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT234348 NZ_LR130531:c2709230-2708925 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3