Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 651171..651898 Replicon chromosome
Accession NZ_CP091662
Organism Escherichia coli strain KFu023

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag L6L71_RS03195 Protein ID WP_000550189.1
Coordinates 651171..651485 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag L6L71_RS03200 Protein ID WP_000560266.1
Coordinates 651482..651898 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L71_RS03175 (647338) 647338..648324 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
L6L71_RS03180 (648403) 648403..649086 - 684 WP_001183043.1 vancomycin high temperature exclusion protein -
L6L71_RS03185 (649163) 649163..649666 - 504 WP_001295542.1 M48 family metallopeptidase -
L6L71_RS03190 (649751) 649751..650887 + 1137 WP_000018681.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
L6L71_RS03195 (651171) 651171..651485 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
L6L71_RS03200 (651482) 651482..651898 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
L6L71_RS03205 (651943) 651943..653961 - 2019 Protein_628 NADPH-dependent 2,4-dienoyl-CoA reductase -
L6L71_RS03210 (654387) 654387..656738 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T234332 WP_000550189.1 NZ_CP091662:651171-651485 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T234332 NZ_LR130527:c2072405-2072127 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT234332 WP_000560266.1 NZ_CP091662:651482-651898 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT234332 NZ_LR130527:c2072115-2071810 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References