Detailed information of TA system TA234315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3049799..3050493
• Replicon: chromosome
• Accession: NZ_CP091659
• Organism: Escherichia coli strain KFu024

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: L6L61_RS15700
• Protein ID: WP_089578406.1
• Coordinates: 3050095..3050493 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: L6L61_RS15695
• Protein ID: WP_000554757.1
• Coordinates: 3049799..3050092 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L61_RS15675 (3045439)	3045439..3045936	+	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-
L6L61_RS15680 (3046152)	3046152..3047864	-	1713	Protein_2887	flagellar biosynthesis protein FlhA	-
L6L61_RS15685 (3047836)	3047836..3048621	+	786	WP_072132640.1	putative lateral flagellar export/assembly protein LafU	-
L6L61_RS15690 (3048692)	3048692..3049747	+	1056	WP_001226164.1	DNA polymerase IV	-
L6L61_RS15695 (3049799)	3049799..3050092	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
L6L61_RS15700 (3050095)	3050095..3050493	+	399	WP_089578406.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
L6L61_RS15705 (3050503)	3050503..3050955	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
L6L61_RS15710 (3051201)	3051201..3051404	+	204	Protein_2893	RtcB family protein	-
L6L61_RS15715 (3051403)	3051403..3051924	+	522	Protein_2894	peptide chain release factor H	-
L6L61_RS15720 (3051981)	3051981..3053438	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
L6L61_RS15725 (3053699)	3053699..3054157	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (3054753)	3054753..3054833	+	81	NuclAT_10	-	-
- (3054753)	3054753..3054833	+	81	NuclAT_10	-	-
- (3054753)	3054753..3054833	+	81	NuclAT_10	-	-
- (3054753)	3054753..3054833	+	81	NuclAT_10	-	-
L6L61_RS15730 (3054249)	3054249..3055493	+	1245	WP_000189541.1	esterase FrsA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15506.85 Da
• Isoelectric Point: 8.0949

>T234315 WP_089578406.1 NZ_CP091659:3050095-3050493 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHFVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T234315 NZ_LR130515:c2365486-2365277 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT234315 WP_000554757.1 NZ_CP091659:3049799-3050092 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT234315 NZ_LR130515:c2365791-2365498 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6