Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3043763..3044304 Replicon chromosome
Accession NZ_CP091659
Organism Escherichia coli strain KFu024

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag L6L61_RS15660 Protein ID WP_000615979.1
Coordinates 3043763..3044041 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag L6L61_RS15665 Protein ID WP_000729704.1
Coordinates 3044044..3044304 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L61_RS15645 (3041345) 3041345..3041923 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
L6L61_RS15650 (3042129) 3042129..3042896 + 768 WP_000333379.1 class II glutamine amidotransferase -
L6L61_RS15655 (3042867) 3042867..3043607 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
L6L61_RS15660 (3043763) 3043763..3044041 - 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
L6L61_RS15665 (3044044) 3044044..3044304 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
L6L61_RS15670 (3044490) 3044490..3045263 + 774 WP_001353765.1 C40 family peptidase -
L6L61_RS15675 (3045439) 3045439..3045936 + 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
L6L61_RS15680 (3046152) 3046152..3047864 - 1713 Protein_2887 flagellar biosynthesis protein FlhA -
L6L61_RS15685 (3047836) 3047836..3048621 + 786 WP_072132640.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 3018404..3045936 27532


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T234314 WP_000615979.1 NZ_CP091659:c3044041-3043763 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T234314 NZ_LR130515:c2345883-2345521 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT234314 WP_000729704.1 NZ_CP091659:c3044304-3044044 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT234314 NZ_LR130515:c2346050-2345880 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References