Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1333651..1334378 Replicon chromosome
Accession NZ_CP091659
Organism Escherichia coli strain KFu024

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag L6L61_RS07325 Protein ID WP_000550189.1
Coordinates 1334064..1334378 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag L6L61_RS07320 Protein ID WP_000560266.1
Coordinates 1333651..1334067 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L61_RS07310 (1328811) 1328811..1331162 + 2352 WP_000695495.1 alpha-glucosidase -
L6L61_RS07315 (1331588) 1331588..1333606 + 2019 WP_113396716.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
L6L61_RS07320 (1333651) 1333651..1334067 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
L6L61_RS07325 (1334064) 1334064..1334378 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
L6L61_RS07330 (1334662) 1334662..1335798 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
L6L61_RS07335 (1335883) 1335883..1336386 + 504 WP_001300832.1 M48 family metallopeptidase -
L6L61_RS07340 (1336463) 1336463..1337155 + 693 WP_096037754.1 vancomycin high temperature exclusion protein -
L6L61_RS07345 (1337234) 1337234..1338220 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T234306 WP_000550189.1 NZ_CP091659:c1334378-1334064 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T234306 NZ_LR130513:c2505488-2505381 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT234306 WP_000560266.1 NZ_CP091659:c1334067-1333651 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT234306 NZ_LR130513:2505304-2505364 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References