Detailed information of TA system TA234293

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 63020..63274
• Replicon: plasmid pKFu024_1
• Accession: NZ_CP091658
• Organism: Escherichia coli strain KFu024

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: L6L61_RS00410
• Protein ID: WP_001312851.1
• Coordinates: 63020..63169 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	63213..63274 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L61_RS00380 (58267)	58267..59178	-	912	WP_000440183.1	carbamate kinase	-
L6L61_RS00385 (59189)	59189..60409	-	1221	WP_000410951.1	arginine deiminase	-
L6L61_RS00390 (61116)	61116..61730	+	615	Protein_77	VENN motif pre-toxin domain-containing protein	-
L6L61_RS00395 (61730)	61730..62176	-	447	Protein_78	plasmid replication initiator RepA	-
L6L61_RS00400 (62169)	62169..62243	-	75	WP_032336874.1	RepA leader peptide Tap	-
L6L61_RS00405 (62479)	62479..62736	-	258	WP_000083833.1	replication regulatory protein RepA	-
L6L61_RS00410 (63020)	63020..63169	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (63213)	63213..63274	+	62	NuclAT_2	-	Antitoxin
- (63213)	63213..63274	+	62	NuclAT_2	-	Antitoxin
- (63213)	63213..63274	+	62	NuclAT_2	-	Antitoxin
- (63213)	63213..63274	+	62	NuclAT_2	-	Antitoxin
L6L61_RS00415 (63413)	63413..63592	-	180	WP_001722474.1	hypothetical protein	-
L6L61_RS00420 (63696)	63696..64312	+	617	Protein_83	IS1-like element IS1A family transposase	-
L6L61_RS00425 (64350)	64350..65921	-	1572	WP_023149734.1	IS66-like element ISCro1 family transposase	-
L6L61_RS00430 (65941)	65941..66288	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
L6L61_RS00435 (66288)	66288..66965	-	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
L6L61_RS00440 (67020)	67020..67109	+	90	Protein_87	IS1 family transposase	-
L6L61_RS00445 (67410)	67410..67622	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / aac(3)-IId / erm(B) / mph(A) / sul1 / qacE / aadA5	iutA / iucD / iucC / iucB / iucA	1..137247	137247

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T234293 WP_001312851.1 NZ_CP091658:c63169-63020 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T234293 NZ_LR130511:c2393470-2393204 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT234293 NZ_CP091658:63213-63274 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin