Detailed information of TA system TA234285

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 19630..20056
• Replicon: plasmid pKMi025_1
• Accession: NZ_CP091654
• Organism: Escherichia coli strain KMi025

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: L6L63_RS24095
• Protein ID: WP_001372321.1
• Coordinates: 19630..19755 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	19832..20056 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L63_RS24055 (15004)	15004..15693	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
L6L63_RS24060 (15880)	15880..16263	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
L6L63_RS24065 (16584)	16584..17186	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
L6L63_RS24070 (17483)	17483..18304	-	822	WP_240725005.1	DUF932 domain-containing protein	-
L6L63_RS24075 (18422)	18422..18709	-	288	WP_000107535.1	hypothetical protein	-
L6L63_RS24080 (18734)	18734..18940	-	207	WP_000547968.1	hypothetical protein	-
L6L63_RS24085 (19010)	19010..19182	+	173	Protein_32	hypothetical protein	-
L6L63_RS24090 (19180)	19180..19410	-	231	WP_071586998.1	hypothetical protein	-
L6L63_RS24095 (19630)	19630..19755	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
L6L63_RS24100 (19697)	19697..19846	-	150	Protein_35	plasmid maintenance protein Mok	-
- (19832)	19832..20056	-	225	NuclAT_0	-	Antitoxin
- (19832)	19832..20056	-	225	NuclAT_0	-	Antitoxin
- (19832)	19832..20056	-	225	NuclAT_0	-	Antitoxin
- (19832)	19832..20056	-	225	NuclAT_0	-	Antitoxin
L6L63_RS24105 (19868)	19868..20056	+	189	WP_001299721.1	hypothetical protein	-
L6L63_RS24110 (20025)	20025..20787	-	763	Protein_37	plasmid SOS inhibition protein A	-
L6L63_RS24115 (20784)	20784..21218	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
L6L63_RS24120 (21273)	21273..21470	-	198	Protein_39	hypothetical protein	-
L6L63_RS24125 (21498)	21498..21731	-	234	WP_000005990.1	DUF905 family protein	-
L6L63_RS24130 (21799)	21799..22338	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
L6L63_RS24135 (22364)	22364..22570	-	207	WP_000275856.1	hypothetical protein	-
L6L63_RS24140 (22640)	22640..22720	+	81	Protein_43	hypothetical protein	-
L6L63_RS24145 (22903)	22903..23072	-	170	Protein_44	hypothetical protein	-
L6L63_RS24150 (23752)	23752..24723	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat	1..128535	128535

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T234285 WP_001372321.1 NZ_CP091654:c19755-19630 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T234285 NZ_LR130509:c2441814-2441548 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT234285 NZ_CP091654:c20056-19832 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin