Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | hok-sok/- |
| Location | 1327..1581 | Replicon | plasmid pKMi025_1 |
| Accession | NZ_CP091654 | ||
| Organism | Escherichia coli strain KMi025 | ||
Toxin (Protein)
| Gene name | srnB | Uniprot ID | G9G1E3 |
| Locus tag | L6L63_RS23945 | Protein ID | WP_001312851.1 |
| Coordinates | 1327..1476 (-) | Length | 50 a.a. |
Antitoxin (RNA)
| Gene name | srnC | ||
| Locus tag | - | ||
| Coordinates | 1520..1581 (+) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| L6L63_RS23925 (5) | 5..487 | - | 483 | WP_001273588.1 | hypothetical protein | - |
| L6L63_RS23930 (480) | 480..527 | - | 48 | WP_229471593.1 | hypothetical protein | - |
| L6L63_RS23935 (518) | 518..769 | + | 252 | WP_240725006.1 | replication protein RepA | - |
| L6L63_RS23940 (786) | 786..1043 | - | 258 | WP_000083845.1 | replication regulatory protein RepA | - |
| L6L63_RS23945 (1327) | 1327..1476 | - | 150 | WP_001312851.1 | Hok/Gef family protein | Toxin |
| - (1520) | 1520..1581 | + | 62 | NuclAT_1 | - | Antitoxin |
| - (1520) | 1520..1581 | + | 62 | NuclAT_1 | - | Antitoxin |
| - (1520) | 1520..1581 | + | 62 | NuclAT_1 | - | Antitoxin |
| - (1520) | 1520..1581 | + | 62 | NuclAT_1 | - | Antitoxin |
| L6L63_RS23950 (1837) | 1837..1911 | - | 75 | Protein_5 | endonuclease | - |
| L6L63_RS23955 (2157) | 2157..2369 | - | 213 | WP_240725003.1 | ANR family transcriptional regulator | - |
| L6L63_RS23960 (2410) | 2410..3087 | + | 678 | WP_001299364.1 | IS66-like element accessory protein TnpA | - |
| L6L63_RS23965 (3087) | 3087..3434 | + | 348 | WP_000624622.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| L6L63_RS23970 (3454) | 3454..5025 | + | 1572 | WP_000381395.1 | IS66-like element ISCro1 family transposase | - |
| L6L63_RS23975 (5212) | 5212..5772 | - | 561 | WP_000139315.1 | fertility inhibition protein FinO | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | sitABCD | iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat | 1..128535 | 128535 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 50 a.a. Molecular weight: 5542.67 Da Isoelectric Point: 8.7678
>T234281 WP_001312851.1 NZ_CP091654:c1476-1327 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK
Download Length: 150 bp
>T234281 NZ_LR130509:c2059765-2059556 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTAATTTAG
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTAATTTAG
Antitoxin
Download Length: 62 bp
>AT234281 NZ_CP091654:1520-1581 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
